Protocol used

Concentration (µg/g FW)

Absorbance ratio

(A₂₆₀/A₂₈₀)

(A₂₆₀/A₂₃₀)

Logemann et al., (1987)

67.09 ± 3.29^e

1.56 ± 0.03^c

1.76 ± 0.05^c

Gasic et al., (2004)

108.80 ± 1.15^c

1.88 ± 0.01^a

1.95 ± 0.03^ab

Salzman et al., (1999)

88.85 ± 2.78^d

1.76 ± 0.02^b

1.50 ± 0.04^d

TRIzol^® (Invitrogen)

125.97 ± 5.59^b

1.65 ± 0.02^bc

1.83 ± 0.02^bc

RNeasy^® (Qiagen)

67.10 ± 2.19^e

1.32 ± 0.02^d

1.72 ± 0.04^c

Modified protocol

520.00 ± 2.60^a

2.02 ± 0.02^a

2.06 ± 0.02^a